Abstract
Miniscope technology has emerged as a powerful tool for in vivo neural imaging, offering researchers unprecedented insights into the dynamics of neural circuits in behaving animals. This article explores recent advancements in Miniscope design, focusing on enhancing imaging performance, versatility, and ease of use. Following, is a comprehensive review of existing Miniscope systems, highlighting their key features, limitations, and applications. Building upon this foundation, novel engineering approaches are proposed to address current challenges and push the boundaries of Miniscope imaging capabilities. Ongoing research on the technology aims to improve in vivo neural imaging by incorporating state-of-the-art optical components and advanced signal processing algorithms. Furthermore, efforts are directed towards miniaturizing the system while maintaining or enhancing imaging quality, allowing for minimally invasive and long-term imaging in freely behaving animals. The ultimate goal is to develop a next-generation imaging platform that empowers researchers with unprecedented capabilities for studying neural dynamics in vivo, thereby advancing our understanding of brain function and neurological disorders.
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